6QUP

Structural signatures in EPR3 define a unique class of plant carbohydrate receptors

Summary for 6QUP

• Entry DOI: 10.2210/pdb6qup/pdb
• Descriptor: LysM type receptor kinase, Nanobody 186 (Nb186), 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: lysm protein, plant protein, nitrogen fixation, plant receptor
• Biological source: Lotus japonicus; More
• Total number of polymer chains: 2
• Total formula weight: 37931.91

Authors

Wong, J.E.,Gysel, K.,Birkefeldt, T.G.,Vinther, M.,Muszynski, A.,Azadi, P.,Laursen, N.S.,Sullivan, J.T.,Ronson, C.W.,Stougaard, J.,Andersen, K.R. (deposition date: 2019-02-28, release date: 2020-08-05, Last modification date: 2024-11-13)

Primary citation

Wong, J.E.M.M.,Gysel, K.,Birkefeldt, T.G.,Vinther, M.,Muszynski, A.,Azadi, P.,Laursen, N.S.,Sullivan, J.T.,Ronson, C.W.,Stougaard, J.,Andersen, K.R.
Structural signatures in EPR3 define a unique class of plant carbohydrate receptors.
Nat Commun, 11:3797-3797, 2020

PubMed Abstract: Receptor-mediated perception of surface-exposed carbohydrates like lipo- and exo-polysaccharides (EPS) is important for non-self recognition and responses to microbial associated molecular patterns in mammals and plants. In legumes, EPS are monitored and can either block or promote symbiosis with rhizobia depending on their molecular composition. To establish a deeper understanding of receptors involved in EPS recognition, we determined the structure of the Lotus japonicus (Lotus) exopolysaccharide receptor 3 (EPR3) ectodomain. EPR3 forms a compact structure built of three putative carbohydrate-binding modules (M1, M2 and LysM3). M1 and M2 have unique βαββ and βαβ folds that have not previously been observed in carbohydrate binding proteins, while LysM3 has a canonical βααβ fold. We demonstrate that this configuration is a structural signature for a ubiquitous class of receptors in the plant kingdom. We show that EPR3 is promiscuous, suggesting that plants can monitor complex microbial communities though this class of receptors.

PubMed: 32732998
DOI: 10.1038/s41467-020-17568-9

Experimental method

X-RAY DIFFRACTION (1.871 Å)