6QUP
Structural signatures in EPR3 define a unique class of plant carbohydrate receptors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.822, 36.083, 72.661 |
Unit cell angles | 90.00, 93.92, 90.00 |
Refinement procedure
Resolution | 72.491 - 1.871 |
R-factor | 0.1666 |
Rwork | 0.163 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ebz |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.491 | 1.940 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.087 | 0.820 |
Number of reflections | 25177 | 2151 |
<I/σ(I)> | 15.96 | 1.94 |
Completeness [%] | 96.9 | 81.1 |
Redundancy | 6.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 292 | 0.1 M Sodium Citrate pH 5.5, 20% PEG 4000, 18% 2-propanol |