6QNZ

Crystal structure of the site-specific DNA nickase N.BspD6I E418A Mutant

6QNZ の概要

• エントリーDOI: 10.2210/pdb6qnz/pdb
• 分子名称: Heterodimeric restriction endonuclease R.BspD6I large subunit, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: nickase, endonuclease, nt.bspd6i, nicking, dna, hydrolase
• 由来する生物種: Bacillus sp. D6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 142289.34

構造登録者

Artyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Gabdulkhakov, A.G.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Zheleznaya, L.A. (登録日: 2019-02-12, 公開日: 2020-03-04, 最終更新日: 2024-01-24)

• 主引用文献: Artyukh, R.I.,Kachalova, G.S.,Yunusova, A.K.,Fatkhullin, B.F.,Atanasov, B.P.,Perevyazova, T.A.,Popov, A.N.,Gabdulkhakov, A.G.,Zheleznaya, L.A.; The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant.; J.Struct.Biol., 210:107508-107508, 2020; PubMed: 32298813; DOI: 10.1016/j.jsb.2020.107508

実験手法

X-RAY DIFFRACTION (2.45 Å)