6QNB

Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3

Summary for 6QNB

• Entry DOI: 10.2210/pdb6qnb/pdb
• Related PRD ID: PRD_900017
• Descriptor: Lysozyme C, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: lysozyme, complex, diffusion, ligand, hydrolase
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 14958.75

Authors

Mehrabi, P.,Schulz, E.C.,Miller, R.J.D. (deposition date: 2019-02-10, release date: 2019-10-02, Last modification date: 2024-11-13)

Primary citation

Mehrabi, P.,Schulz, E.C.,Agthe, M.,Horrell, S.,Bourenkov, G.,von Stetten, D.,Leimkohl, J.P.,Schikora, H.,Schneider, T.R.,Pearson, A.R.,Tellkamp, F.,Miller, R.J.D.
Liquid application method for time-resolved analyses by serial synchrotron crystallography.
Nat.Methods, 16:979-982, 2019

PubMed Abstract: We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.

PubMed: 31527838
DOI: 10.1038/s41592-019-0553-1

Experimental method

X-RAY DIFFRACTION (1.7 Å)