6QNB
Liquid Application Method for time-resolved Analyses (LAMA) by serial synchrotron crystallography, Lysozyme with GlcNAc3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2018-09-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.210, 79.210, 38.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.802 - 1.700 |
R-factor | 0.1946 |
Rwork | 0.194 |
R-free | 0.21080 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.834 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.802 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 13934 | |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.9 | |
Redundancy | 148.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 20 mM NaAcetate pH 4.7, 50 mM NaCl |