6QJO
DNA containing both right- and left-handed parallel-stranded G-quadruplexes
Summary for 6QJO
| Entry DOI | 10.2210/pdb6qjo/pdb |
| Descriptor | DNA (28-MER), POTASSIUM ION (3 entities in total) |
| Functional Keywords | g-quadruplex left-handed right-handed, dna |
| Biological source | synthetic construct |
| Total number of polymer chains | 4 |
| Total formula weight | 36177.12 |
| Authors | Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Schmitt, E.,Mechulam, Y.,Phan, A.T. (deposition date: 2019-01-24, release date: 2019-07-03, Last modification date: 2024-01-24) |
| Primary citation | Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Mechulam, Y.,Schmitt, E.,Phan, A.T. NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes. Nucleic Acids Res., 47:8272-8281, 2019 Cited by PubMed Abstract: Analogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering. PubMed: 31216034DOI: 10.1093/nar/gkz349 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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