6QJO
DNA containing both right- and left-handed parallel-stranded G-quadruplexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.120, 39.270, 53.550 |
| Unit cell angles | 90.04, 93.03, 89.95 |
Refinement procedure
| Resolution | 39.270 - 1.800 |
| R-factor | 0.1717 |
| Rwork | 0.164 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u5m |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.355 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 22501 | 3079 |
| <I/σ(I)> | 10.45 | 5.8 |
| Completeness [%] | 95.9 | 88 |
| Redundancy | 4.1 | 2.72 |
| CC(1/2) | 0.990 | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 60% MPD |
