6QFA

CryoEM structure of a beta3K279T GABA(A)R homomer in complex with histamine and megabody Mb25

Summary for 6QFA

Entry DOI	10.2210/pdb6qfa/pdb
EMDB information	4542
Descriptor	Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit beta-3, Outer membrane protein,Outer membrane protein,Outer membrane protein,Outer membrane protein,Uncharacterized protein,Uncharacterized protein,Mb-c7HopQ-Nb25, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
Functional Keywords	megabody, membrane protein, protein engineering, cryo-em
Biological source	Homo sapiens (Human) More
Total number of polymer chains	10
Total formula weight	485236.80
Authors	Uchanski, T.,Masiulis, S.,Fischer, B.,Kalichuk, V.,Wohlkoening, A.,Zoegg, T.,Remaut, H.,Vranken, W.,Aricescu, A.R.,Pardon, E.,Steyaert, J. (deposition date: 2019-01-09, release date: 2021-08-04)
Primary citation	Uchanski, T.,Masiulis, S.,Fischer, B.,Kalichuk, V.,Lopez-Sanchez, U.,Zarkadas, E.,Weckener, M.,Sente, A.,Ward, P.,Wohlkonig, A.,Zogg, T.,Remaut, H.,Naismith, J.H.,Nury, H.,Vranken, W.,Aricescu, A.R.,Pardon, E.,Steyaert, J. Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM. Nat.Methods, 18:60-68, 2021 Cited by PubMed: 33408403 DOI: 10.1038/s41592-020-01001-6 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.49 Å)

Structure validation