6Q97

Structure of tmRNA SmpB bound in A site of E. coli 70S ribosome

これはPDB形式変換不可エントリーです。

6Q97 の概要

• エントリーDOI: 10.2210/pdb6q97/pdb
• 関連するPDBエントリー: 6Q95
• EMDBエントリー: 4476
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L4, 50S ribosomal protein L5, ... (63 entities in total)
• 機能のキーワード: tmrna smpb trans-translation translation, ribosome
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2338335.08

構造登録者

Rae, C.D. (登録日: 2018-12-17, 公開日: 2019-03-13, 最終更新日: 2024-04-24)

主引用文献

Rae, C.D.,Gordiyenko, Y.,Ramakrishnan, V.
How a circularized tmRNA moves through the ribosome.
Science, 363:740-744, 2019

PubMed Abstract: During trans-translation, transfer-messenger RNA (tmRNA) and small protein B (SmpB) together rescue ribosomes stalled on a truncated mRNA and tag the nascent polypeptide for degradation. We used cryo-electron microscopy to determine the structures of three key states of the tmRNA-SmpB-ribosome complex during trans translation at resolutions of 3.7 to 4.4 angstroms. The results show how tmRNA and SmpB act specifically on stalled ribosomes and how the circularized complex moves through the ribosome, enabling translation to switch from the old defective message to the reading frame on tmRNA.

PubMed: 30765567
DOI: 10.1126/science.aav9370

実験手法

ELECTRON MICROSCOPY (3.9 Å)