6Q5V

1-Cys SiPrx, a Prx6-family 1-Cys peroxiredoxin of the thermoacidophilic archaeon Sulfolobus islandicus

Replaces:  6GWWReplaces:  6FEU

Summary for 6Q5V

• Entry DOI: 10.2210/pdb6q5v/pdb
• Descriptor: Peroxiredoxin (1 entity in total)
• Functional Keywords: archaea, peroxiredoxin, sulfolobus islandicus, oxidoreductase
• Biological source: Sulfolobus islandicus (strain REY15A)
• Total number of polymer chains: 10
• Total formula weight: 246766.54

Authors

Stroobants, S.,Maes, D.,Peeters, E.,van Molle, I. (deposition date: 2018-12-09, release date: 2019-05-29, Last modification date: 2024-01-24)

Primary citation

Stroobants, S.,Van Molle, I.,Saidi, Q.,Jonckheere, K.,Maes, D.,Peeters, E.
Structure of the Prx6-subfamily 1-Cys peroxiredoxin from Sulfolobus islandicus.
Acta Crystallogr.,Sect.F, 75:428-434, 2019

PubMed Abstract: Aerobic thermoacidophilic archaea belonging to the genus Sulfolobus harbor peroxiredoxins, thiol-dependent peroxidases that assist in protecting the cells from oxidative damage. Here, the crystal structure of the 1-Cys peroxiredoxin from Sulfolobus islandicus, named 1-Cys SiPrx, is presented. A 2.75 Å resolution data set was collected from a crystal belonging to space group P222, with unit-cell parameters a = 86.8, b = 159.1, c = 189.3 Å, α = β = γ = 90°. The structure was solved by molecular replacement using the homologous Aeropyrum pernix peroxiredoxin (ApPrx) structure as a search model. In the crystal structure, 1-Cys SiPrx assembles into a ring-shaped decamer composed of five homodimers. This quaternary structure corresponds to the oligomeric state of the protein in solution, as observed by size-exclusion chromatography. 1-Cys SiPrx harbors only a single cysteine, which is the peroxidatic cysteine, and lacks both of the cysteines that are highly conserved in the C-terminal arm domain in other archaeal Prx6-subfamily proteins such as ApPrx and that are involved in the association of dimers into higher-molecular-weight decamers and dodecamers. It is thus concluded that the Sulfolobus Prx6-subfamily protein undergoes decamerization independently of arm-domain cysteines.

PubMed: 31204689
DOI: 10.1107/S2053230X19006472

Experimental method

X-RAY DIFFRACTION (2.747 Å)