6Q5V
1-Cys SiPrx, a Prx6-family 1-Cys peroxiredoxin of the thermoacidophilic archaeon Sulfolobus islandicus
Replaces: 6GWWReplaces: 6FEUExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2016-12-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.789, 159.060, 189.316 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.186 - 2.747 |
R-factor | 0.2137 |
Rwork | 0.213 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a2v |
RMSD bond length | 0.010 |
RMSD bond angle | 1.603 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.186 | 2.862 |
High resolution limit [Å] | 2.747 | 2.747 |
Rmerge | 0.095 | 0.957 |
Rmeas | 0.105 | 1.053 |
Rpim | 0.044 | 0.433 |
Number of reflections | 51577 | 2580 |
<I/σ(I)> | 12.7 | 33 |
Completeness [%] | 74.6 | 33 |
Redundancy | 5.5 | 5.7 |
CC(1/2) | 0.999 | 0.776 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 15% w/v PEG1500 and 0.1M MgCl2 in a 10 mMTris buffer |