6Q3T

Structure of Protease1 from Pyrococcus horikoshii at room temperature in ChipX microfluidic device

Summary for 6Q3T

• Entry DOI: 10.2210/pdb6q3t/pdb
• Descriptor: Deglycase PH1704 (2 entities in total)
• Functional Keywords: chipx, nucleation, xo4, crystallophore, room temperature, counterdiffusion, hydrolase
• Biological source: Pyrococcus horikoshii
• Total number of polymer chains: 3
• Total formula weight: 55428.93

Authors

de Wijn, R.,Engilberge, S.,Olieric, V.,Girard, E.,Sauter, C. (deposition date: 2018-12-04, release date: 2019-05-01, Last modification date: 2024-01-24)

Primary citation

de Wijn, R.,Hennig, O.,Roche, J.,Engilberge, S.,Rollet, K.,Fernandez-Millan, P.,Brillet, K.,Betat, H.,Morl, M.,Roussel, A.,Girard, E.,Mueller-Dieckmann, C.,Fox, G.C.,Olieric, V.,Gavira, J.A.,Lorber, B.,Sauter, C.
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.
Iucrj, 6:454-464, 2019

PubMed Abstract: Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

PubMed: 31098026
DOI: 10.1107/S2052252519003622

Experimental method

X-RAY DIFFRACTION (2.15 Å)