6Q3T

Structure of Protease1 from Pyrococcus horikoshii at room temperature in ChipX microfluidic device

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0036524	molecular_function	protein deglycase activity
B	0005737	cellular_component	cytoplasm
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0036524	molecular_function	protein deglycase activity
C	0005737	cellular_component	cytoplasm
C	0006508	biological_process	proteolysis
C	0008233	molecular_function	peptidase activity
C	0036524	molecular_function	protein deglycase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Nucleophile => ECO:0000305|PubMed:11114201

Chain	Residue	Details
A	CYS100
B	CYS100
C	CYS100

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site_id	SWS_FT_FI2
Number of Residues	3
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU00608

Chain	Residue	Details
A	HIS101
B	HIS101
C	HIS101

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
A	GLY70	electrostatic stabiliser
A	ARG71	electrostatic stabiliser
A	GLU74	electrostatic stabiliser, modifies pKa
A	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	HIS101	electrostatic stabiliser, proton acceptor, proton donor
A	TYR120	electrostatic stabiliser

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site_id	MCSA2
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
B	GLY70	electrostatic stabiliser
B	ARG71	electrostatic stabiliser
B	GLU74	electrostatic stabiliser, modifies pKa
B	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	HIS101	electrostatic stabiliser, proton acceptor, proton donor
B	TYR120	electrostatic stabiliser

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site_id	MCSA3
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
C	GLY70	electrostatic stabiliser
C	ARG71	electrostatic stabiliser
C	GLU74	electrostatic stabiliser, modifies pKa
C	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	HIS101	electrostatic stabiliser, proton acceptor, proton donor
C	TYR120	electrostatic stabiliser

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