6Q3T

Structure of Protease1 from Pyrococcus horikoshii at room temperature in ChipX microfluidic device

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005737	cellular_component	cytoplasm
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0016787	molecular_function	hydrolase activity
A	0036524	molecular_function	protein deglycase activity
B	0005737	cellular_component	cytoplasm
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0016787	molecular_function	hydrolase activity
B	0036524	molecular_function	protein deglycase activity
C	0005737	cellular_component	cytoplasm
C	0006508	biological_process	proteolysis
C	0008233	molecular_function	peptidase activity
C	0016787	molecular_function	hydrolase activity
C	0036524	molecular_function	protein deglycase activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	495
Details	Domain: {"description":"PfpI endopeptidase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00608","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"11114201","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00608","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
A	GLY70	electrostatic stabiliser
A	ARG71	electrostatic stabiliser
A	GLU74	electrostatic stabiliser, modifies pKa
A	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	HIS101	electrostatic stabiliser, proton acceptor, proton donor
A	TYR120	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
B	GLY70	electrostatic stabiliser
B	ARG71	electrostatic stabiliser
B	GLU74	electrostatic stabiliser, modifies pKa
B	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	HIS101	electrostatic stabiliser, proton acceptor, proton donor
B	TYR120	electrostatic stabiliser

---

site_id	MCSA3
Number of Residues	6
Details	M-CSA 964

Chain	Residue	Details
C	GLY70	electrostatic stabiliser
C	ARG71	electrostatic stabiliser
C	GLU74	electrostatic stabiliser, modifies pKa
C	CYS100	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	HIS101	electrostatic stabiliser, proton acceptor, proton donor
C	TYR120	electrostatic stabiliser

---