6PIS

Mouse two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure

6PIS の概要

• エントリーDOI: 10.2210/pdb6pis/pdb
• 分子名称: Potassium channel subfamily K member 4, ANTIBODY FAB FRAGMENT LIGHT CHAIN, ANTIBODY FAB FRAGMENT HEAVY CHAIN, ... (4 entities in total)
• 機能のキーワード: potassium ion channel, ion transport, membrane protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 162810.46

構造登録者

Brohawn, S.G. (登録日: 2019-06-27, 公開日: 2019-12-25, 最終更新日: 2024-10-23)

主引用文献

Brohawn, S.G.,Wang, W.,Handler, A.,Campbell, E.B.,Schwarz, J.R.,MacKinnon, R.
The mechanosensitive ion channel TRAAK is localized to the mammalian node of Ranvier.
Elife, 8:-, 2019

PubMed Abstract: TRAAK is a membrane tension-activated K channel that has been associated through behavioral studies to mechanical nociception. We used specific monoclonal antibodies in mice to show that TRAAK is localized exclusively to nodes of Ranvier, the action potential propagating elements of myelinated nerve fibers. Approximately 80 percent of myelinated nerve fibers throughout the central and peripheral nervous system contain TRAAK in what is likely an all-nodes or no-nodes per axon fashion. TRAAK is not observed at the axon initial segment where action potentials are first generated. We used polyclonal antibodies, the TRAAK inhibitor RU2 and node clamp amplifiers to demonstrate the presence and functional properties of TRAAK in rat nerve fibers. TRAAK contributes to the 'leak' K current in mammalian nerve fiber conduction by hyperpolarizing the resting membrane potential, thereby increasing Na channel availability for action potential propagation. We speculate on why nodes of Ranvier contain a mechanosensitive K channel.

PubMed: 31674909
DOI: 10.7554/eLife.50403

実験手法

X-RAY DIFFRACTION (2.77 Å)