6P78

queuine lyase from Clostridium spiroforme bound to SAM and queuine

Summary for 6P78

DescriptorQueuine lyase, IRON/SULFUR CLUSTER, 2-amino-5-({[(1S,4S,5S)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-1,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one, ... (5 entities in total)
Functional Keywordsmetal ion binding, 4 iron 4 sulfur cluster binding, radical sam enzyme, c-n lyase activity, lyase
Biological source[Clostridium] spiroforme DSM 1552
Total number of polymer chains1
Total molecular weight30961.44
Authors
Almo, S.C.,Grove, T.L. (deposition date: 2019-06-05, release date: 2019-09-18, Last modification date: 2019-12-18)
Primary citation
Yuan, Y.,Zallot, R.,Grove, T.L.,Payan, D.J.,Martin-Verstraete, I.,Sepic, S.,Balamkundu, S.,Neelakandan, R.,Gadi, V.K.,Liu, C.F.,Swairjo, M.A.,Dedon, P.C.,Almo, S.C.,Gerlt, J.A.,de Crecy-Lagard, V.
Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens.
Proc.Natl.Acad.Sci.USA, 116:19126-19135, 2019
PubMed: 31481610 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1909604116
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.726 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.19920 1.4%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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