6P78

queuine lyase from Clostridium spiroforme bound to SAM and queuine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Queuine lyase	polymer	251	29934.1	1	UniProt (B1C2R2) In PDB	[Clostridium] spiroforme DSM 1552	Radical SAM domain protein
2	A	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
3	A	2-amino-5-({[(1S,4S,5S)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-1,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one	non-polymer		277.3	1	Chemie (QUG)
4	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5		water	water		18.0	221	Chemie (HOH)

Sequence modifications

A: 1 - 229 (UniProt: B1C2R2)

PDB	External Database	Details
Met -21	-	initiating methionine
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Leu -9	-	expression tag
Gly -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29934.1
Non-Polymers*	Number of molecules	3
	Total formula weight	1027.4
All*	Total formula weight	30961.4

*Water molecules are not included.