6P5I
Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1)
This is a non-PDB format compatible entry.
Summary for 6P5I
| Entry DOI | 10.2210/pdb6p5i/pdb |
| Related | 6P4G 6P4H |
| EMDB information | 20248 20249 20255 |
| Descriptor | 18S rRNA, eS8, uS4, ... (81 entities in total) |
| Functional Keywords | israeli acute paralysis virus, internal ribosome entry site, ires, small ribosomal subunit, 40s, large ribosomal subunit, 60s, 80s, ribosome |
| Biological source | Israeli acute paralysis virus More |
| Total number of polymer chains | 81 |
| Total formula weight | 3411882.11 |
| Authors | Acosta-Reyes, F.J.,Neupane, R.,Frank, J.,Fernandez, I.S. (deposition date: 2019-05-30, release date: 2019-09-18, Last modification date: 2020-02-26) |
| Primary citation | Acosta-Reyes, F.,Neupane, R.,Frank, J.,Fernandez, I.S. The Israeli acute paralysis virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs. Embo J., 38:e102226-e102226, 2019 Cited by PubMed Abstract: Colony collapse disorder (CCD) is a multi-faceted syndrome decimating bee populations worldwide, and a group of viruses of the widely distributed Dicistroviridae family have been identified as a causing agent of CCD. This family of viruses employs non-coding RNA sequences, called internal ribosomal entry sites (IRESs), to precisely exploit the host machinery for viral protein production. Using single-particle cryo-electron microscopy (cryo-EM), we have characterized how the IRES of Israeli acute paralysis virus (IAPV) intergenic region captures and redirects translating ribosomes toward viral RNA messages. We reconstituted two in vitro reactions targeting a pre-translocation and a post-translocation state of the IAPV-IRES in the ribosome, allowing us to identify six structures using image processing classification methods. From these, we reconstructed the trajectory of IAPV-IRES from the early small subunit recruitment to the final post-translocated state in the ribosome. An early commitment of IRES/ribosome complexes for global pre-translocation mimicry explains the high efficiency observed for this IRES. Efforts directed toward fighting CCD by targeting the IAPV-IRES using RNA-interference technology are underway, and the structural framework presented here may assist in further refining these approaches. PubMed: 31609474DOI: 10.15252/embj.2019102226 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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