6P5I

Structure of a mammalian 80S ribosome in complex with the Israeli Acute Paralysis Virus IRES (Class 1)

これはPDB形式変換不可エントリーです。

6P5I の概要

• エントリーDOI: 10.2210/pdb6p5i/pdb
• 関連するPDBエントリー: 6P4G 6P4H
• EMDBエントリー: 20248 20249 20255
• 分子名称: 18S rRNA, eS8, uS4, ... (81 entities in total)
• 機能のキーワード: israeli acute paralysis virus, internal ribosome entry site, ires, small ribosomal subunit, 40s, large ribosomal subunit, 60s, 80s, ribosome
• 由来する生物種: Israeli acute paralysis virus; 詳細
• タンパク質・核酸の鎖数: 81
• 化学式量合計: 3411882.11

構造登録者

Acosta-Reyes, F.J.,Neupane, R.,Frank, J.,Fernandez, I.S. (登録日: 2019-05-30, 公開日: 2019-09-18, 最終更新日: 2020-02-26)

主引用文献

Acosta-Reyes, F.,Neupane, R.,Frank, J.,Fernandez, I.S.
The Israeli acute paralysis virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs.
Embo J., 38:e102226-e102226, 2019

PubMed Abstract: Colony collapse disorder (CCD) is a multi-faceted syndrome decimating bee populations worldwide, and a group of viruses of the widely distributed Dicistroviridae family have been identified as a causing agent of CCD. This family of viruses employs non-coding RNA sequences, called internal ribosomal entry sites (IRESs), to precisely exploit the host machinery for viral protein production. Using single-particle cryo-electron microscopy (cryo-EM), we have characterized how the IRES of Israeli acute paralysis virus (IAPV) intergenic region captures and redirects translating ribosomes toward viral RNA messages. We reconstituted two in vitro reactions targeting a pre-translocation and a post-translocation state of the IAPV-IRES in the ribosome, allowing us to identify six structures using image processing classification methods. From these, we reconstructed the trajectory of IAPV-IRES from the early small subunit recruitment to the final post-translocated state in the ribosome. An early commitment of IRES/ribosome complexes for global pre-translocation mimicry explains the high efficiency observed for this IRES. Efforts directed toward fighting CCD by targeting the IAPV-IRES using RNA-interference technology are underway, and the structural framework presented here may assist in further refining these approaches.

PubMed: 31609474
DOI: 10.15252/embj.2019102226

実験手法

ELECTRON MICROSCOPY (3.1 Å)