6OPA

Crystal structure of bovine Fab NC-Cow1 in complex with HIV-1 BG505 SOSIP.664, and human Fabs 35022 and PGT128

6OPA の概要

• エントリーDOI: 10.2210/pdb6opa/pdb
• 分子名称: Envelope glycoprotein gp160, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (15 entities in total)
• 機能のキーワード: hiv-1 broadly neutralizing antibody, viral protein-immune system complex, immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 229340.44

構造登録者

Stanfield, R.L.,Wilson, I.A. (登録日: 2019-04-24, 公開日: 2020-04-01, 最終更新日: 2024-11-13)

主引用文献

Stanfield, R.L.,Berndsen, Z.T.,Huang, R.,Sok, D.,Warner, G.,Torres, J.L.,Burton, D.R.,Ward, A.B.,Wilson, I.A.,Smider, V.V.
Structural basis of broad HIV neutralization by a vaccine-induced cow antibody.
Sci Adv, 6:eaba0468-eaba0468, 2020

PubMed Abstract: Potent broadly neutralizing antibodies (bnAbs) to HIV have been very challenging to elicit by vaccination in wild-type animals. Here, by x-ray crystallography, cryo-electron microscopy, and site-directed mutagenesis, we structurally and functionally elucidate the mode of binding of a potent bnAb (NC-Cow1) elicited in cows by immunization with the HIV envelope (Env) trimer BG505 SOSIP.664. The exceptionally long (60 residues) third complementarity-determining region of the heavy chain (CDR H3) of NC-Cow1 forms a mini domain (knob) on an extended stalk that navigates through the dense glycan shield on Env to target a small footprint on the gp120 CD4 receptor binding site with no contact of the other CDRs to the rest of the Env trimer. These findings illustrate, in molecular detail, how an unusual vaccine-induced cow bnAb to HIV Env can neutralize with high potency and breadth.

PubMed: 32518821
DOI: 10.1126/sciadv.aba0468

実験手法

X-RAY DIFFRACTION (4.084 Å)