6OET

Cryo-EM structure of mouse RAG1/2 STC complex

6OET の概要

• エントリーDOI: 10.2210/pdb6oet/pdb
• 関連するPDBエントリー: 6OEM 6OEN 6OEO 6OEP 6OEQ 6OER 6OES
• EMDBエントリー: 20030 20031 20032 20033 20034 20035 20036 20037
• 分子名称: V(D)J recombination-activating protein 1, V(D)J recombination-activating protein 2, DNA (50-MER), ... (9 entities in total)
• 機能のキーワード: v(d)j recombination, dna transposition, rag, scid, recombination, recombination-dna complex, recombination/dna
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 422496.41

構造登録者

Chen, X.,Cui, Y.,Zhou, Z.H.,Yang, W.,Gellert, M. (登録日: 2019-03-27, 公開日: 2020-01-22, 最終更新日: 2025-05-21)

主引用文献

Chen, X.,Cui, Y.,Wang, H.,Zhou, Z.H.,Gellert, M.,Yang, W.
How mouse RAG recombinase avoids DNA transposition.
Nat.Struct.Mol.Biol., 27:127-133, 2020

PubMed Abstract: The RAG1-RAG2 recombinase (RAG) cleaves DNA to initiate V(D)J recombination, but RAG also belongs to the RNH-type transposase family. To learn how RAG-catalyzed transposition is inhibited in developing lymphocytes, we determined the structure of a DNA-strand transfer complex of mouse RAG at 3.1-Å resolution. The target DNA is a T form (T for transpositional target), which contains two >80° kinks towards the minor groove, only 3 bp apart. RAG2, a late evolutionary addition in V(D)J recombination, appears to enforce the sharp kinks and additional inter-segment twisting in target DNA and thus attenuates unwanted transposition. In contrast to strand transfer complexes of genuine transposases, where severe kinks occur at the integration sites of target DNA and thus prevent the reverse reaction, the sharp kink with RAG is 1 bp away from the integration site. As a result, RAG efficiently catalyzes the disintegration reaction that restores the RSS (donor) and target DNA.

PubMed: 32015553
DOI: 10.1038/s41594-019-0366-z

実験手法

ELECTRON MICROSCOPY (3.4 Å)