Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6OET

Cryo-EM structure of mouse RAG1/2 STC complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002250	biological_process	adaptive immune response
A	0002331	biological_process	pre-B cell allelic exclusion
A	0003677	molecular_function	DNA binding
A	0004519	molecular_function	endonuclease activity
A	0004842	molecular_function	ubiquitin-protein transferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0006310	biological_process	DNA recombination
A	0006325	biological_process	chromatin organization
A	0008270	molecular_function	zinc ion binding
A	0008542	biological_process	visual learning
A	0016740	molecular_function	transferase activity
A	0030183	biological_process	B cell differentiation
A	0033077	biological_process	T cell differentiation in thymus
A	0033151	biological_process	V(D)J recombination
A	0042393	molecular_function	histone binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043029	biological_process	T cell homeostasis
A	0043565	molecular_function	sequence-specific DNA binding
A	0045580	biological_process	regulation of T cell differentiation
A	0045582	biological_process	positive regulation of T cell differentiation
A	0046872	molecular_function	metal ion binding
A	0048538	biological_process	thymus development
A	0051865	biological_process	protein autoubiquitination
A	0061630	molecular_function	ubiquitin protein ligase activity
A	0070233	biological_process	negative regulation of T cell apoptotic process
A	0070244	biological_process	negative regulation of thymocyte apoptotic process
A	2000822	biological_process	regulation of behavioral fear response
B	0002313	biological_process	mature B cell differentiation involved in immune response
B	0002326	biological_process	B cell lineage commitment
B	0002331	biological_process	pre-B cell allelic exclusion
B	0002358	biological_process	B cell homeostatic proliferation
B	0002360	biological_process	T cell lineage commitment
B	0003677	molecular_function	DNA binding
B	0003682	molecular_function	chromatin binding
B	0005515	molecular_function	protein binding
B	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
B	0005547	molecular_function	phosphatidylinositol-3,4,5-trisphosphate binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0006310	biological_process	DNA recombination
B	0006325	biological_process	chromatin organization
B	0008270	molecular_function	zinc ion binding
B	0030183	biological_process	B cell differentiation
B	0030217	biological_process	T cell differentiation
B	0033077	biological_process	T cell differentiation in thymus
B	0033085	biological_process	negative regulation of T cell differentiation in thymus
B	0033151	biological_process	V(D)J recombination
B	0035064	molecular_function	methylated histone binding
B	0035091	molecular_function	phosphatidylinositol binding
B	0035265	biological_process	organ growth
B	0042742	biological_process	defense response to bacterium
B	0043325	molecular_function	phosphatidylinositol-3,4-bisphosphate binding
B	0046622	biological_process	positive regulation of organ growth
B	0046872	molecular_function	metal ion binding
B	0080025	molecular_function	phosphatidylinositol-3,5-bisphosphate binding
B	1904155	biological_process	DN2 thymocyte differentiation
C	0002250	biological_process	adaptive immune response
C	0002331	biological_process	pre-B cell allelic exclusion
C	0003677	molecular_function	DNA binding
C	0004519	molecular_function	endonuclease activity
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0006310	biological_process	DNA recombination
C	0006325	biological_process	chromatin organization
C	0008270	molecular_function	zinc ion binding
C	0008542	biological_process	visual learning
C	0016740	molecular_function	transferase activity
C	0030183	biological_process	B cell differentiation
C	0033077	biological_process	T cell differentiation in thymus
C	0033151	biological_process	V(D)J recombination
C	0042393	molecular_function	histone binding
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043029	biological_process	T cell homeostasis
C	0043565	molecular_function	sequence-specific DNA binding
C	0045580	biological_process	regulation of T cell differentiation
C	0045582	biological_process	positive regulation of T cell differentiation
C	0046872	molecular_function	metal ion binding
C	0048538	biological_process	thymus development
C	0051865	biological_process	protein autoubiquitination
C	0061630	molecular_function	ubiquitin protein ligase activity
C	0070233	biological_process	negative regulation of T cell apoptotic process
C	0070244	biological_process	negative regulation of thymocyte apoptotic process
C	2000822	biological_process	regulation of behavioral fear response
D	0002313	biological_process	mature B cell differentiation involved in immune response
D	0002326	biological_process	B cell lineage commitment
D	0002331	biological_process	pre-B cell allelic exclusion
D	0002358	biological_process	B cell homeostatic proliferation
D	0002360	biological_process	T cell lineage commitment
D	0003677	molecular_function	DNA binding
D	0003682	molecular_function	chromatin binding
D	0005515	molecular_function	protein binding
D	0005546	molecular_function	phosphatidylinositol-4,5-bisphosphate binding
D	0005547	molecular_function	phosphatidylinositol-3,4,5-trisphosphate binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0006310	biological_process	DNA recombination
D	0006325	biological_process	chromatin organization
D	0008270	molecular_function	zinc ion binding
D	0030183	biological_process	B cell differentiation
D	0030217	biological_process	T cell differentiation
D	0033077	biological_process	T cell differentiation in thymus
D	0033085	biological_process	negative regulation of T cell differentiation in thymus
D	0033151	biological_process	V(D)J recombination
D	0035064	molecular_function	methylated histone binding
D	0035091	molecular_function	phosphatidylinositol binding
D	0035265	biological_process	organ growth
D	0042742	biological_process	defense response to bacterium
D	0043325	molecular_function	phosphatidylinositol-3,4-bisphosphate binding
D	0046622	biological_process	positive regulation of organ growth
D	0046872	molecular_function	metal ion binding
D	0080025	molecular_function	phosphatidylinositol-3,5-bisphosphate binding
D	1904155	biological_process	DN2 thymocyte differentiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue CA A 1101

Chain	Residue
A	ASP600
A	GLY601
F	DG30
F	DC31

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 1102

Chain	Residue
A	CYS727
A	CYS730
A	HIS937
A	HIS942

site_id	AC3
Number of Residues	3
Details	binding site for residue CA C 1101

Chain	Residue
C	GLY601
G	DC42
C	ASP600

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN C 1102

Chain	Residue
C	CYS727
C	CYS730
C	HIS937
C	HIS942

Functional Information from PROSITE/UniProt

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. DTVYILGGHSL

Chain	Residue	Details
B	ASP214-LEU224

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CkHlFCriCI

Chain	Residue	Details
A	CYS305-ILE314

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	136
Details	ZN_FING: PHD-type; atypical

Chain	Residue	Details
B	TRP416-ILE484
D	TRP416-ILE484

site_id	SWS_FT_FI2
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:15964836, ECO:0000269\|PubMed:18025461

Chain	Residue	Details
B	CYS419
D	CYS423
D	CYS446
D	HIS452
D	HIS455
D	CYS458
D	CYS478
D	HIS481
B	CYS423
B	CYS446
B	HIS452
B	HIS455
B	CYS458
B	CYS478
B	HIS481
D	CYS419

site_id	SWS_FT_FI3
Number of Residues	134
Details	DNA_BIND: NBD => ECO:0000255\|PROSITE-ProRule:PRU00820

Chain	Residue	Details
A	GLY389-GLN456
C	GLY389-GLN456

site_id	SWS_FT_FI4
Number of Residues	22
Details	BINDING: BINDING => ECO:0000269\|PubMed:9228952

Chain	Residue	Details
A	CYS266
A	CYS325
A	CYS328
C	CYS266
C	HIS270
C	CYS290
C	CYS293
C	HIS295
C	CYS305
C	HIS307
C	CYS310
A	HIS270
C	CYS313
C	CYS325
C	CYS328
A	CYS290
A	CYS293
A	HIS295
A	CYS305
A	HIS307
A	CYS310
A	CYS313

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU01101, ECO:0000269\|PubMed:9228952

Chain	Residue	Details
A	CYS355
A	CYS360
A	HIS372
A	HIS376
C	CYS355
C	CYS360
C	HIS372
C	HIS376

site_id	SWS_FT_FI6
Number of Residues	6
Details	BINDING: BINDING => ECO:0000305

Chain	Residue	Details
A	ASP600
A	ASP708
A	GLN962
C	ASP600
C	ASP708
C	GLN962

site_id	SWS_FT_FI7
Number of Residues	2
Details	SITE: Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site

Chain	Residue	Details
A	TRP893
C	TRP893

site_id	SWS_FT_FI8
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:14671314

Chain	Residue	Details
A	LYS233
C	LYS233

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)