6O0Z

Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium

Summary for 6O0Z

Entry DOI	10.2210/pdb6o0z/pdb
EMDB information	0585
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, single guide RNA, target strand DNA, ... (4 entities in total)
Functional Keywords	crispr, cas9, nuclease, genome editing, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	216673.61
Authors	Zhu, X.,Clarke, R.,Puppala, A.K.,Chittori, S.,Merk, A.,Merrill, B.J.,Simonovic, M.,Subramaniam, S. (deposition date: 2019-02-17, release date: 2019-07-10, Last modification date: 2024-03-20)
Primary citation	Zhu, X.,Clarke, R.,Puppala, A.K.,Chittori, S.,Merk, A.,Merrill, B.J.,Simonovic, M.,Subramaniam, S. Cryo-EM structures reveal coordinated domain motions that govern DNA cleavage by Cas9. Nat.Struct.Mol.Biol., 26:679-685, 2019 Cited by PubMed: 31285607 DOI: 10.1038/s41594-019-0258-2 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.3 Å)

Structure validation