6O0Z
Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1372 | 158986.1 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) In PDB | Streptococcus pyogenes | SpCas9,SpyCas9 |
2 | B | single guide RNA | polymer | 102 | 33059.7 | 1 | synthetic construct | ||
3 | C | target strand DNA | polymer | 40 | 12175.8 | 1 | synthetic construct | ||
4 | D | non-target strand DNA | polymer | 40 | 12452.0 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1368 (UniProt: Q99ZW2)
PDB | External Database | Details |
---|---|---|
Gly -3 | - | expression tag |
Ala -2 | - | expression tag |
Ala -1 | - | expression tag |
Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 216673.6 | |
All* | Total formula weight | 216673.6 |