6NL0

Ternary complex crystal structure of K289M variant of DNA polymerase Beta with "hot-spot sequence" with beta-gamma CF2 analogue of dGTP

Summary for 6NL0

• Entry DOI: 10.2210/pdb6nl0/pdb
• Descriptor: DNA (5'-D(*CP*CP*GP*AP*AP*CP*AP*AP*GP*CP*AP*TP*CP*AP*GP*C)-3'), DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*TP*(2DT))-3'), DNA (5'-D(P*TP*TP*CP*GP*G)-3'), ... (9 entities in total)
• Functional Keywords: dna polymerase beta, conformational change, enzyme mechanism, lfer, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 48320.81

Authors

Batra, V.K.,Wilson, S.H. (deposition date: 2019-01-07, release date: 2020-01-08, Last modification date: 2024-03-13)

• Primary citation: Batra, V.K.,Alnajjar, K.S.,Sweasy, J.B.,McKenna, C.E.,Goodman, M.F.,Wilson, S.H.; Revealing an Internal Stabilization Deficiency in the DNA Polymerase beta K289M Cancer Variant through the Combined Use of Chemical Biology and X-ray Crystallography.; Biochemistry, 59:955-963, 2020; PubMed: 31999437; DOI: 10.1021/acs.biochem.9b01072

Experimental method

X-RAY DIFFRACTION (1.97 Å)