6NIT

Human Argonaute2-miR-122 bound to a target RNA with four central mismatches (bu4)

Summary for 6NIT

• Entry DOI: 10.2210/pdb6nit/pdb
• Descriptor: Protein argonaute-2, RNA (5'-R(P*UP*GP*GP*AP*GP*UP*GP*UP*GP*AP*CP*AP*AP*UP*GP*GP*UP*GP*UP*UP*U)-3'), RNA (5'-R(P*AP*AP*CP*AP*CP*CP*AP*UP*CP*CP*AP*AP*CP*AP*CP*UP*CP*CP*AP*AP*A)-3') (3 entities in total)
• Functional Keywords: rna-binding protein, microrna, target directed microrna decay, hydrolase, rna binding protein, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 222853.38

Authors

Sheu-Gruttadauria, J.,MacRae, I.J. (deposition date: 2018-12-31, release date: 2019-08-07, Last modification date: 2024-03-13)

Primary citation

Sheu-Gruttadauria, J.,Pawlica, P.,Klum, S.M.,Wang, S.,Yario, T.A.,Schirle Oakdale, N.T.,Steitz, J.A.,MacRae, I.J.
Structural Basis for Target-Directed MicroRNA Degradation.
Mol.Cell, 75:1243-1255.e7, 2019

PubMed Abstract: MicroRNAs (miRNAs) broadly regulate gene expression through association with Argonaute (Ago), which also protects miRNAs from degradation. However, miRNA stability is known to vary and is regulated by poorly understood mechanisms. A major emerging process, termed target-directed miRNA degradation (TDMD), employs specialized target RNAs to selectively bind to miRNAs and induce their decay. Here, we report structures of human Ago2 (hAgo2) bound to miRNAs and TDMD-inducing targets. miRNA and target form a bipartite duplex with an unpaired flexible linker. hAgo2 cannot physically accommodate the RNA, causing the duplex to bend at the linker and display the miRNA 3' end for enzymatic attack. Altering 3' end display by changing linker flexibility, changing 3' end complementarity, or mutationally inducing 3' end release impacts TDMD efficiency, leading to production of distinct 3'-miRNA isoforms in cells. Our results uncover the mechanism driving TDMD and reveal 3' end display as a key determinant regulating miRNA activity via 3' remodeling and/or degradation.

PubMed: 31353209
DOI: 10.1016/j.molcel.2019.06.019

Experimental method

X-RAY DIFFRACTION (3.8 Å)