6NIT
Human Argonaute2-miR-122 bound to a target RNA with four central mismatches (bu4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.712, 137.086, 154.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.301 - 3.800 |
R-factor | 0.2453 |
Rwork | 0.243 |
R-free | 0.29410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.821 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.560 | 4.100 |
High resolution limit [Å] | 3.790 | 3.790 |
Rmerge | 0.283 | 1.068 |
Rpim | 0.112 | 0.430 |
Number of reflections | 23149 | 4605 |
<I/σ(I)> | 5.8 | 1.9 |
Completeness [%] | 98.9 | 97 |
Redundancy | 7.3 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG3350, 50mM Tris pH8, 20mM MgCl2, 75mM Phenol |