6NHV
Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold
Summary for 6NHV
| Entry DOI | 10.2210/pdb6nhv/pdb |
| EMDB information | 9373 9374 |
| Descriptor | superfolder GFP, DARP14 - Subunit B, Subunit A-DARPin (3 entities in total) |
| Functional Keywords | protein engineering, symmetric scaffold, small protein cryo-em, display platform, biosynthetic protein |
| Biological source | Aequorea victoria More |
| Total number of polymer chains | 7 |
| Total formula weight | 173816.20 |
| Authors | Liu, Y.,Huynh, D.,Yeates, T.O. (deposition date: 2018-12-24, release date: 2019-05-08, Last modification date: 2024-11-13) |
| Primary citation | Liu, Y.,Huynh, D.T.,Yeates, T.O. A 3.8 angstrom resolution cryo-EM structure of a small protein bound to an imaging scaffold. Nat Commun, 10:1864-1864, 2019 Cited by PubMed Abstract: Proteins smaller than about 50 kDa are currently too small to be imaged at high resolution by cryo-electron microscopy (cryo-EM), leaving most protein molecules in the cell beyond the reach of this powerful structural technique. Here we use a designed protein scaffold to bind and symmetrically display 12 copies of a small 26 kDa protein, green fluorescent protein (GFP). We show that the bound cargo protein is held rigidly enough to visualize it at a resolution of 3.8 Å by cryo-EM, where specific structural features of the protein are visible. The designed scaffold is modular and can be modified through modest changes in its amino acid sequence to bind and display diverse proteins for imaging, thus providing a general method to break through the lower size limitation in cryo-EM. PubMed: 31015551DOI: 10.1038/s41467-019-09836-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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