6NHV

Single particle reconstruction of DARPin and its bound GFP on a symmetric scaffold

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	superfolder GFP	polymer	236	26623.9	1		Aequorea victoria
2	B, C, G (N, O, X)	DARP14 - Subunit B	polymer	131	14346.3	3	UniProt (Q9I2D8) Pfam (PF02962)	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
3	D, E, F (R, S, T)	Subunit A-DARPin	polymer	319	34717.8	3		Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)

Sequence modifications

N, O, X: 1 - 123 (UniProt: Q9I2D8)

PDB	External Database	Details
Lys 27	Ala 27	engineered mutation
Ile 74	Ala 74	engineered mutation
Thr 78	Gln 78	engineered mutation
Leu 79	Ala 79	engineered mutation
Ala 82	Glu 82	engineered mutation
Ala 86	Glu 86	engineered mutation
Glu 90	Gly 90	engineered mutation
Leu 112	Ala 112	engineered mutation
Leu 124	-	expression tag
Glu 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	173816.2
All*	Total formula weight	173816.2

*Water molecules are not included.