6N8N

Cryo-EM structure of Lsg1-engaged (LE) pre-60S ribosomal subunit

This is a non-PDB format compatible entry.

Summary for 6N8N

• Entry DOI: 10.2210/pdb6n8n/pdb
• Related: 6N8J 6N8K 6N8L 6N8M 6N8O
• EMDB information: 0369 0370 0371 0372 0373 0374
• Descriptor: Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 60S ribosomal protein L4-A, 60S ribosomal protein L5, ... (48 entities in total)
• Functional Keywords: ribosome biogenesis, nmd3, peptidyl transferase center, ribosome
• Biological source: Saccharomyces cerevisiae S288C; More
• Total number of polymer chains: 48
• Total formula weight: 2136130.38

Authors

Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W. (deposition date: 2018-11-29, release date: 2019-03-13, Last modification date: 2024-10-30)

Primary citation

Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W.
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Nat Commun, 10:958-958, 2019

PubMed Abstract: The catalytic activity of the ribosome is mediated by RNA, yet proteins are essential for the function of the peptidyl transferase center (PTC). In eukaryotes, final assembly of the PTC occurs in the cytoplasm by insertion of the ribosomal protein Rpl10 (uL16). We determine structures of six intermediates in late nuclear and cytoplasmic maturation of the large subunit that reveal a tightly-choreographed sequence of protein and RNA rearrangements controlling the insertion of Rpl10. We also determine the structure of the biogenesis factor Yvh1 and show how it promotes assembly of the P stalk, a critical element for recruitment of GTPases that drive translation. Together, our structures provide a blueprint for final assembly of a functional ribosome.

PubMed: 30814529
DOI: 10.1038/s41467-019-08880-0

Experimental method

ELECTRON MICROSCOPY (3.8 Å)