6N8N

Cryo-EM structure of Lsg1-engaged (LE) pre-60S ribosomal subunit

This is a large structure.

Summary for 6N8N

Related6N8J 6N8K 6N8L 6N8M 6N8O
EMDB information0373 0369 0370 0371 0372 0374
DescriptorSaccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 5S rRNA, 5.8S rRNA, ... (48 entities in total)
Functional Keywordsribosome biogenesis, nmd3, peptidyl transferase center, ribosome
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Total number of polymer chains48
Total molecular weight2136130.38
Authors
Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W. (deposition date: 2018-11-29, release date: 2019-03-13)
Primary citation
Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W.
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Nat Commun, 10:958-958, 2019
PubMed: 30814529 (PDB entries with the same primary citation)
DOI: 10.1038/s41467-019-08880-0
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone4 0.5% 1.1% 0.48MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 6n8n
no rotation
Molmil generated image of 6n8n
rotated about x axis by 90°
Molmil generated image of 6n8n
rotated about y axis by 90°