6N8K

Cryo-EM structure of early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit

This is a large structure.

Summary for 6N8K

Related6N8J 6N8L 6N8M 6N8N 6N8O
EMDB information0370 0369 0371 0372 0373 0374
DescriptorRibosomal Protein uL1, 60S ribosomal export protein NMD3, 60S ribosomal protein L2-A, ... (50 entities in total)
Functional Keywordsribosome biogenesis, nmd3, peptidyl transferase center, ribosome
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Total number of polymer chains48
Total molecular weight2131039.83
Authors
Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W. (deposition date: 2018-11-29, release date: 2019-03-13)
Primary citation
Zhou, Y.,Musalgaonkar, S.,Johnson, A.W.,Taylor, D.W.
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Nat Commun, 10:958-958, 2019
PubMed: 30814529 (PDB entries with the same primary citation)
DOI: 10.1038/s41467-019-08880-0
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone4 0.4% 1.2% 0.47MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 6n8k
no rotation
Molmil generated image of 6n8k
rotated about x axis by 90°
Molmil generated image of 6n8k
rotated about y axis by 90°