6N8K
Cryo-EM structure of early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (s) | Ribosomal Protein uL1 | polymer | 217 | 18485.7 | 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||
| 2 | B (v) | 60S ribosomal export protein NMD3 | polymer | 518 | 59167.0 | 1 | UniProt (P38861) Pfam (PF04981) Pfam (PF21193) Pfam (PF21192) | Saccharomyces cerevisiae S288C | Nonsense-mediated mRNA decay protein 3 |
| 3 | C (A) | 60S ribosomal protein L2-A | polymer | 254 | 27463.6 | 1 | UniProt (P0CX45) Pfam (PF00181) Pfam (PF03947) | Saccharomyces cerevisiae S288C | L5,Large ribosomal subunit protein uL2-A,RP8,YL6 |
| 4 | D (a) | 60S ribosomal protein L28 | polymer | 149 | 16761.7 | 1 | UniProt (P02406) Pfam (PF00828) | Saccharomyces cerevisiae S288C | L27a,L29,Large ribosomal subunit protein uL15,RP44,RP62,YL24 |
| 5 | E (B) | 60S ribosomal protein L3 | polymer | 387 | 43850.8 | 1 | UniProt (P14126) Pfam (PF00297) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein uL3,Maintenance of killer protein 8,RP1,Trichodermin resistance protein,YL1 |
| 6 | F (b) | Nucleolar GTP-binding protein 1 | polymer | 647 | 74531.2 | 1 | UniProt (Q02892) Pfam (PF17835) Pfam (PF06858) Pfam (PF08155) | Saccharomyces cerevisiae S288C | |
| 7 | G (C) | 60S ribosomal protein L4-A | polymer | 362 | 39159.1 | 1 | UniProt (P10664) Pfam (PF00573) Pfam (PF14374) | Saccharomyces cerevisiae S288C | L2,Large ribosomal subunit protein uL4-A,RP2,YL2 |
| 8 | H (c) | 60S ribosomal protein L30 | polymer | 105 | 11430.4 | 1 | UniProt (P14120) Pfam (PF01248) | Saccharomyces cerevisiae S288C | L32,Large ribosomal subunit protein eL30,RP73,YL38 |
| 9 | I (d) | 60S ribosomal protein L31-A | polymer | 113 | 12980.2 | 1 | UniProt (P0C2H8) Pfam (PF01198) | Saccharomyces cerevisiae S288C | L34,Large ribosomal subunit protein eL31-A,YL28 |
| 10 | J (E) | 60S ribosomal protein L6-A | polymer | 176 | 20000.6 | 1 | UniProt (Q02326) Pfam (PF01159) | Saccharomyces cerevisiae S288C | L17,Large ribosomal subunit protein eL6-A,RP18,YL16 |
| 11 | K (e) | 60S ribosomal protein L32 | polymer | 130 | 14809.4 | 1 | UniProt (P38061) Pfam (PF01655) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL32 |
| 12 | L (F) | 60S ribosomal protein L7-A | polymer | 244 | 27686.3 | 1 | UniProt (P05737) Pfam (PF00327) Pfam (PF08079) | Saccharomyces cerevisiae S288C | L6,Large ribosomal subunit protein uL30-A,RP11,YL8 |
| 13 | M (f) | 60S ribosomal protein L33-A | polymer | 107 | 12177.1 | 1 | UniProt (P05744) Pfam (PF01247) | Saccharomyces cerevisiae S288C | L37,Large ribosomal subunit protein eL33-A,RP47,YL37 |
| 14 | N (G) | 60S ribosomal protein L8-A | polymer | 256 | 28175.8 | 1 | UniProt (P17076) Pfam (PF01248) | Saccharomyces cerevisiae S288C | L4,L4-2,L7a-1,Large ribosomal subunit protein eL8-A,Maintenance of killer protein 7,RP6,YL5 |
| 15 | O (g) | 60S ribosomal protein L34-A | polymer | 121 | 13673.2 | 1 | UniProt (P87262) Pfam (PF01199) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL34-A |
| 16 | P (I) | Bud site selection protein 20 | polymer | 166 | 18547.0 | 1 | UniProt (Q08004) Pfam (PF12171) | Saccharomyces cerevisiae S288C | |
| 17 | Q (H) | 60S ribosomal protein L9-A | polymer | 191 | 21605.1 | 1 | UniProt (P05738) Pfam (PF00347) | Saccharomyces cerevisiae S288C | L8,Large ribosomal subunit protein uL6-A,RP24,YL11 |
| 18 | R (h) | 60S ribosomal protein L35-A | polymer | 120 | 13942.6 | 1 | UniProt (P0CX84) Pfam (PF00831) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein uL29-A |
| 19 | S (i) | 60S ribosomal protein L36-A | polymer | 100 | 11151.3 | 1 | UniProt (P05745) Pfam (PF01158) | Saccharomyces cerevisiae S288C | L39,Large ribosomal subunit protein eL36-A,YL39 |
| 20 | T (j) | 60S ribosomal protein L37-A | polymer | 88 | 9877.4 | 1 | UniProt (P49166) Pfam (PF01907) | Saccharomyces cerevisiae S288C | L43,Large ribosomal subunit protein eL37-A,YL35,YP55 |
| 21 | U (k) | 60S ribosomal protein L38 | polymer | 78 | 8845.6 | 1 | UniProt (P49167) Pfam (PF01781) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL38 |
| 22 | V (L) | 60S ribosomal protein L13-A | polymer | 199 | 22604.2 | 1 | UniProt (Q12690) Pfam (PF01294) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL13-A |
| 23 | W (l) | 60S ribosomal protein L39 | polymer | 51 | 6358.6 | 1 | UniProt (P04650) Pfam (PF00832) | Saccharomyces cerevisiae S288C | L46,Large ribosomal subunit protein eL39,YL40 |
| 24 | X (M) | 60S ribosomal protein L14-A | polymer | 138 | 15195.1 | 1 | UniProt (P36105) Pfam (PF00467) Pfam (PF01929) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL14-A |
| 25 | Y (p) | 60S ribosomal protein L43-A | polymer | 92 | 10113.0 | 1 | UniProt (P0CX25) Pfam (PF01780) | Saccharomyces cerevisiae S288C | L37a,Large ribosomal subunit protein eL43-A,YL35 |
| 26 | Z (N) | 60S ribosomal protein L15-A | polymer | 204 | 24482.4 | 1 | UniProt (P05748) Pfam (PF00827) | Saccharomyces cerevisiae S288C | L13,Large ribosomal subunit protein eL15-A,RP15R,YL10,YP18 |
| 27 | AA (u) | Ribosome biogenesis protein RLP24 | polymer | 199 | 24027.7 | 1 | UniProt (Q07915) Pfam (PF01246) | Saccharomyces cerevisiae S288C | Ribosomal protein L24-like |
| 28 | BA (O) | 60S ribosomal protein L16-A | polymer | 199 | 22247.2 | 1 | UniProt (P26784) Pfam (PF00572) | Saccharomyces cerevisiae S288C | L13a,L21,Large ribosomal subunit protein uL13-A,RP22,YL15 |
| 29 | CA (P) | 60S ribosomal protein L17-A | polymer | 184 | 20589.5 | 1 | UniProt (P05740) Pfam (PF00237) | Saccharomyces cerevisiae S288C | L20A,Large ribosomal subunit protein uL22-A,YL17 |
| 30 | DA (Q) | 60S ribosomal protein L18-A | polymer | 186 | 20609.3 | 1 | UniProt (P0CX49) Pfam (PF17135) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL18-A,RP28 |
| 31 | EA (R) | 60S ribosomal protein L19-A | polymer | 189 | 21762.3 | 1 | UniProt (P0CX82) Pfam (PF01280) | Saccharomyces cerevisiae S288C | L23,Large ribosomal subunit protein eL19-A,RP15L,RP33,YL14 |
| 32 | FA (S) | 60S ribosomal protein L20-A | polymer | 172 | 20478.9 | 1 | UniProt (P0CX23) Pfam (PF01775) | Saccharomyces cerevisiae S288C | L18a,Large ribosomal subunit protein eL20-A |
| 33 | GA (T) | 60S ribosomal protein L21-A | polymer | 160 | 18279.3 | 1 | UniProt (Q02753) Pfam (PF01157) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL21-A |
| 34 | HA (U) | 60S ribosomal protein L22-A | polymer | 121 | 13711.4 | 1 | UniProt (P05749) Pfam (PF01776) | Saccharomyces cerevisiae S288C | L1c,Large ribosomal subunit protein eL22-A,RP4,YL31 |
| 35 | IA (V) | 60S ribosomal protein L23-A | polymer | 137 | 14494.0 | 1 | UniProt (P0CX41) Pfam (PF00238) | Saccharomyces cerevisiae S288C | L17a,Large ribosomal subunit protein uL14-A,YL32 |
| 36 | JA (W) | Ribosome assembly factor MRT4 | polymer | 236 | 27098.0 | 1 | UniProt (P33201) Pfam (PF00466) Pfam (PF17777) | Saccharomyces cerevisiae S288C | mRNA turnover protein 4 |
| 37 | KA (X) | 60S ribosomal protein L25 | polymer | 142 | 15787.6 | 1 | UniProt (P04456) Pfam (PF03939) Pfam (PF00276) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein uL23,RP16L,YL25,YP42' |
| 38 | LA (Y) | 60S ribosomal protein L26-A | polymer | 127 | 14265.8 | 1 | UniProt (P05743) Pfam (PF16906) Pfam (PF00467) | Saccharomyces cerevisiae S288C | L33,Large ribosomal subunit protein uL24-A,YL33 |
| 39 | MA (y) | Eukaryotic translation initiation factor 6 | polymer | 245 | 26476.6 | 1 | UniProt (Q12522) Pfam (PF01912) | Saccharomyces cerevisiae S288C | eIF-6 |
| 40 | NA (Z) | 60S ribosomal protein L27-A | polymer | 136 | 15568.4 | 1 | UniProt (P0C2H6) Pfam (PF01777) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL27-A |
| 41 | OA (z) | UPF0642 protein YBL028C | polymer | 106 | 12435.4 | 1 | UniProt (P38202) Pfam (PF10338) | Saccharomyces cerevisiae S288C | |
| 42 | PA (D) | 60S ribosomal protein L5 | polymer | 297 | 33764.8 | 1 | UniProt (P26321) Pfam (PF17144) Pfam (PF14204) | Saccharomyces cerevisiae S288C | L1,L1a,Large ribosomal subunit protein uL18,Ribosomal 5S RNA-binding protein,YL3 |
| 43 | QA (J) | 60S ribosomal protein L11-A | polymer | 174 | 19755.7 | 1 | UniProt (P0C0W9) Pfam (PF00281) Pfam (PF00673) | Saccharomyces cerevisiae S288C | L16,Large ribosomal subunit protein uL5-A,RP39,YL22 |
| 44 | RA (q) | 60S ribosomal protein L42-A | polymer | 106 | 12246.7 | 1 | UniProt (P0CX27) Pfam (PF00935) | Saccharomyces cerevisiae S288C | L41,Large ribosomal subunit protein eL42-A,YL27,YP44 |
| 45 | SA (o) | 60S ribosomal protein L29 | polymer | 59 | 6691.9 | 1 | UniProt (P05747) Pfam (PF01779) | Saccharomyces cerevisiae S288C | Large ribosomal subunit protein eL29,YL43 |
| 46 | TA (1) | Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA | polymer | 3396 | 1097493.9 | 1 | GenBank (831416132) | Saccharomyces cerevisiae S288C | |
| 47 | UA (2) | 5S rRNA | polymer | 121 | 38951.1 | 1 | GenBank (1329886537) | Saccharomyces cerevisiae S288C | |
| 48 | VA (3) | 5.8S rRNA | polymer | 158 | 50682.9 | 1 | GenBank (1489318609) | Saccharomyces cerevisiae S288C | |
| 49 | WA (b) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 50 | XA (b) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 48 |
| Total formula weight | 2130492.3 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 2131039.8 |
