6MXF

MicroED structure of thiostrepton at 1.9 A resolution

Summary for 6MXF

• Entry DOI: 10.2210/pdb6mxf/pdb
• EMDB information: 9282 9283 9284 9285 9286 9287 9288 9289 9290 9291 9292
• Related PRD ID: PRD_000223
• Descriptor: Thiostrepton (2 entities in total)
• Functional Keywords: antibiotic
• Biological source: Streptomyces azureus
• Total number of polymer chains: 1
• Total formula weight: 1805.98

Authors

Jones, C.G.,Martynowycz, M.W.,Hattne, J.,Fulton, T.,Stoltz, B.M.,Rodriguez, J.A.,Nelson, H.M.,Gonen, T. (deposition date: 2018-10-30, release date: 2018-11-21, Last modification date: 2023-11-15)

Primary citation

Jones, C.G.,Martynowycz, M.W.,Hattne, J.,Fulton, T.J.,Stoltz, B.M.,Rodriguez, J.A.,Nelson, H.M.,Gonen, T.
The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination.
ACS Cent Sci, 4:1587-1592, 2018

PubMed Abstract: In the many scientific endeavors that are driven by organic chemistry, unambiguous identification of small molecules is of paramount importance. Over the past 50 years, NMR and other powerful spectroscopic techniques have been developed to address this challenge. While almost all of these techniques rely on inference of connectivity, the unambiguous determination of a small molecule's structure requires X-ray and/or neutron diffraction studies. In practice, however, X-ray crystallography is rarely applied in routine organic chemistry due to intrinsic limitations of both the analytes and the technique. Here we report the use of the electron cryo-microscopy (cryoEM) method microcrystal electron diffraction (MicroED) to provide routine and unambiguous structural determination of small organic molecules. From simple powders, with minimal sample preparation, we could collect high-quality MicroED data from nanocrystals (∼100 nm, ∼10 g) resulting in atomic resolution (<1 Å) crystal structures in minutes.

PubMed: 30555912
DOI: 10.1021/acscentsci.8b00760

Experimental method

ELECTRON CRYSTALLOGRAPHY (1.91 Å)