6MXF
MicroED structure of thiostrepton at 1.9 A resolution
Experimental procedure
| Collection date | 2018-10-03 |
| Wavelength(s) | 0.0251 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 26.219, 26.219, 27.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.990 - 1.910 |
| R-factor | 0.19276 |
| Rwork | 0.190 |
| R-free | 0.21798 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e9w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.760 |
| Data reduction software | MOSFLM (7.2.2) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | MOLREP (11.6.04) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.990 | 2.130 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.236 | 0.320 |
| Rmeas | 0.251 | |
| Rpim | 0.084 | |
| Number of reflections | 686 | |
| <I/σ(I)> | 5.1 | 3.4 |
| Completeness [%] | 78.6 | 40.3 |
| Redundancy | 8.1 | 4.9 |
| CC(1/2) | 0.985 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
