6MGH

X-ray structure of monomeric near-infrared fluorescent protein miRFP670nano

Summary for 6MGH

• Entry DOI: 10.2210/pdb6mgh/pdb
• Descriptor: miRFP670nano, 3-[2-[(~{Z})-[5-[(~{Z})-[(3~{S},4~{R})-3-ethenyl-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-pyrrol-2-ylidene]methyl]-5-[(~{Z})-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid, GLYCEROL, ... (5 entities in total)
• Functional Keywords: near-infrared fluorescent protein, gaf domain, cyanobacteriochrome fp, fluorescent protein
• Biological source: Acaryochloris marina
• Total number of polymer chains: 8
• Total formula weight: 155745.27

Authors

Pletnev, S. (deposition date: 2018-09-13, release date: 2018-12-19, Last modification date: 2024-10-23)

Primary citation

Oliinyk, O.S.,Shemetov, A.A.,Pletnev, S.,Shcherbakova, D.M.,Verkhusha, V.V.
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing.
Nat Commun, 10:279-279, 2019

PubMed Abstract: From a single domain of cyanobacteriochrome (CBCR) we developed a near-infrared (NIR) fluorescent protein (FP), termed miRFP670nano, with excitation at 645 nm and emission at 670 nm. This is the first CBCR-derived NIR FP evolved to efficiently bind endogenous biliverdin chromophore and brightly fluoresce in mammalian cells. miRFP670nano is a monomer with molecular weight of 17 kDa that is 2-fold smaller than bacterial phytochrome (BphP)-based NIR FPs and 1.6-fold smaller than GFP-like FPs. Crystal structure of the CBCR-based NIR FP with biliverdin reveals a molecular basis of its spectral and biochemical properties. Unlike BphP-derived NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an internal protein tag. miRFP670nano is an effective FRET donor for red-shifted NIR FPs, enabling engineering NIR FRET biosensors spectrally compatible with GFP-like FPs and blue-green optogenetic tools. miRFP670nano unlocks a new source of diverse CBCR templates for NIR FPs.

PubMed: 30655515
DOI: 10.1038/s41467-018-08050-8

Experimental method

X-RAY DIFFRACTION (1.95 Å)