6MGH
X-ray structure of monomeric near-infrared fluorescent protein miRFP670nano
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-01 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.598, 72.124, 136.698 |
Unit cell angles | 90.00, 129.99, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.950 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 2.415 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.097 | 0.045 | 0.557 |
Rmeas | 0.113 | 0.053 | 0.661 |
Rpim | 0.058 | 0.027 | 0.353 |
Total number of observations | 376413 | ||
Number of reflections | 100484 | 10354 | 9875 |
<I/σ(I)> | 7.8 | ||
Completeness [%] | 98.5 | 99.3 | 97.6 |
Redundancy | 3.7 | 3.7 | 3.3 |
CC(1/2) | 0.996 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1M Na Acetate pH 4.0, 10mM EDTA, 10% isopropanol, 22% PEG 6000 |