6ME1

Crystal structure of the clade B isolate B41 mutant fusion peptide (residues 512-521) in complex with VRC34.01

Summary for 6ME1

• Entry DOI: 10.2210/pdb6me1/pdb
• Related: 6MCO 6MDT
• Descriptor: VRC34.01 Fab heavy chain, VRC34.01 Fab light chain, Envelope glycoprotein gp160, ... (4 entities in total)
• Functional Keywords: viral protein, hiv, envelope, fusion peptide, hiv-1 gp41, immune system, neutralizing antibodies, complex, viral protein-immune system complex
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 6
• Total formula weight: 97336.68

Authors

Kumar, S.,Sarkar, A.,Wilson, I.A. (deposition date: 2018-09-05, release date: 2019-02-27, Last modification date: 2024-10-23)

Primary citation

Kumar, S.,Sarkar, A.,Pugach, P.,Sanders, R.W.,Moore, J.P.,Ward, A.B.,Wilson, I.A.
Capturing the inherent structural dynamics of the HIV-1 envelope glycoprotein fusion peptide.
Nat Commun, 10:763-763, 2019

PubMed Abstract: The N-terminal fusion peptide (FP) of the human immunodeficiency virus (HIV)-1 envelope glycoprotein (Env) gp41 subunit plays a critical role in cell entry. However, capturing the structural flexibility in the unbound FP is challenging in the native Env trimer. Here, FP conformational isomerism is observed in two crystal structures of a soluble clade B transmitted/founder virus B41 SOSIP.664 Env with broadly neutralizing antibodies (bNAbs) PGT124 and 35O22 to aid in crystallization and that are not specific for binding to the FP. Large rearrangements in the FP and fusion peptide proximal region occur around M530, which remains anchored in the tryptophan clasp (gp41 W623, W628, W631) in the B41 Env prefusion state. Further, we redesigned the FP at position 518 to reinstate the bNAb VRC34.01 epitope. These findings provide further structural evidence for the dynamic nature of the FP and how a bNAb epitope can be restored during vaccine design.

PubMed: 30770829
DOI: 10.1038/s41467-019-08738-5

Experimental method

X-RAY DIFFRACTION (1.97 Å)