6M32

Cryo-EM structure of FMO-RC complex from green sulfur bacteria

Summary for 6M32

• Entry DOI: 10.2210/pdb6m32/pdb
• EMDB information: 30069
• Descriptor: Bacteriochlorophyll a protein, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE, 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE, ... (13 entities in total)
• Functional Keywords: photosynthetic reaction center, green sulfur bacteria, energy transfer, photosynthesis
• Biological source: Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS); More
• Total number of polymer chains: 7
• Total formula weight: 380925.76

Authors

Chen, J.H.,Zhang, X. (deposition date: 2020-03-02, release date: 2020-11-25, Last modification date: 2025-04-09)

Primary citation

Chen, J.H.,Wu, H.,Xu, C.,Liu, X.C.,Huang, Z.,Chang, S.,Wang, W.,Han, G.,Kuang, T.,Shen, J.R.,Zhang, X.
Architecture of the photosynthetic complex from a green sulfur bacterium.
Science, 370:-, 2020

PubMed Abstract: The photosynthetic apparatus of green sulfur bacteria (GSB) contains a peripheral antenna chlorosome, light-harvesting Fenna-Matthews-Olson proteins (FMO), and a reaction center (GsbRC). We used cryo-electron microscopy to determine a 2.7-angstrom structure of the FMO-GsbRC supercomplex from The GsbRC binds considerably fewer (bacterio)chlorophylls [(B)Chls] than other known type I RCs do, and the organization of (B)Chls is similar to that in photosystem II. Two BChl layers in GsbRC are not connected by Chls, as seen in other RCs, but associate with two carotenoid derivatives. Relatively long distances of 22 to 33 angstroms were observed between BChls of FMO and GsbRC, consistent with the inefficient energy transfer between these entities. The structure contains common features of both type I and type II RCs and provides insight into the evolution of photosynthetic RCs.

PubMed: 33214250
DOI: 10.1126/science.abb6350

Experimental method

ELECTRON MICROSCOPY (2.7 Å)