6LE3

Crystal structure of gluconate 5-dehydrogenase from Lentibacter algarum

Summary for 6LE3

Entry DOI	10.2210/pdb6le3/pdb
Descriptor	Gluconate 5-dehydrogenase, ACETYL GROUP (3 entities in total)
Functional Keywords	gluconate 5-dehydrogenase, biosynthetic protein
Biological source	Lentibacter algarum
Total number of polymer chains	4
Total formula weight	108894.86
Authors	Yuan, H. (deposition date: 2019-11-23, release date: 2020-05-13, Last modification date: 2023-11-22)
Primary citation	Tian, D.,Fu, X.,Cao, W.,Yuan, H. Crystal structure of gluconate 5-dehydrogenase from Lentibacter algarum. Acta Crystallogr.,Sect.F, 76:228-234, 2020 Cited by PubMed Abstract: Gluconate 5-dehydrogenase (Ga5DH; EC 1.1.1.69) from Lentibacter algarum (LaGa5DH) was recombinantly expressed in Escherichia coli and purified to homogeneity. The protein was crystallized and the crystal structure was solved at 2.1 Å resolution. The crystal belonged to the monoclinic system, with space group P1 and unit-cell parameters a = 55.42, b = 55.48, c = 79.16 Å, α = 100.51, β = 105.66, γ = 97.99°. The structure revealed LaGaDH to be a tetramer, with each subunit consisting of six α-helices and three antiparallel β-hairpins. LaGa5DH has high structural similarity to other Ga5DH proteins, demonstrating that this enzyme is highly conserved. PubMed: 32356525 DOI: 10.1107/S2053230X20005336 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation