6LE3
Crystal structure of gluconate 5-dehydrogenase from Lentibacter algarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.988 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.424, 55.484, 79.158 |
| Unit cell angles | 100.51, 105.66, 97.99 |
Refinement procedure
| Resolution | 37.400 - 2.100 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uf0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.111 | 0.407 |
| Rpim | 0.070 | 0.272 |
| Number of reflections | 49082 | 2093 |
| <I/σ(I)> | 10.34 | 3.36 |
| Completeness [%] | 96.7 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2 M Ammonium Chloride, 0.1 M Sodium Acetate, pH 4.6 |
