6L9J

Structure of yeast Snf5 and Swi3 subcomplex

Summary for 6L9J

• Entry DOI: 10.2210/pdb6l9j/pdb
• Descriptor: SWI/SNF chromatin-remodeling complex subunit SNF5, SWI/SNF complex subunit SWI3, GLYCEROL, ... (4 entities in total)
• Functional Keywords: complex, gene regulation
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); More
• Total number of polymer chains: 12
• Total formula weight: 280932.36

Authors

Long, J.,Zhou, H. (deposition date: 2019-11-10, release date: 2020-11-11, Last modification date: 2024-03-27)

Primary citation

Zhou, H.,Chen, G.,Dong, C.,Zhao, X.,Shen, Z.,Chen, F.,Liu, B.,Long, J.
Snf5 and Swi3 subcomplex formation is required for SWI/SNF complex function in yeast.
Biochem.Biophys.Res.Commun., 526:934-940, 2020

PubMed Abstract: The SWI/SNF chromatin remodeling complex, which alters nucleosome positions by either evicting histones or sliding nucleosomes on DNA, is highly conserved from yeast to humans, and 20% of all human cancers have mutations in various subunits of the SWI/SNF complex. Here, we reported the crystal structure of the yeast Snf5-Swi3 subcomplex at a resolution of 2.65 Å. Our results showed that the Snf5-Swi3 subcomplex assembles into a heterotrimer with one Snf5 molecule bound to two distinct Swi3 molecules. In addition, we demonstrated that Snf5-Swi3 subcomplex formation is required for SWI/SNF function in yeast. These findings shed light on the important role of the Snf5-Swi3 subcomplex in the assembly and functional integrity of the SWI/SNF complex.

PubMed: 32284172
DOI: 10.1016/j.bbrc.2020.03.169

Experimental method

X-RAY DIFFRACTION (2.642 Å)