6L9J
Structure of yeast Snf5 and Swi3 subcomplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 205.522, 146.459, 154.329 |
Unit cell angles | 90.00, 131.20, 90.00 |
Refinement procedure
Resolution | 48.621 - 2.642 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.27950 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.889 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.640 | 2.650 |
Rpim | 0.076 | 0.365 |
Number of reflections | 99168 | 4909 |
<I/σ(I)> | 8.79 | |
Completeness [%] | 99.3 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100mM HEPES (pH 7.8), 230mM Li2SO4, 20%-24% (w/v) PEG 3350 |