6L9H

The Human Telomeric Nucleosome Displays Distinct Structural and Dynamic Properties

Summary for 6L9H

• Entry DOI: 10.2210/pdb6l9h/pdb
• Descriptor: Histone H3.1, Histone H4, Histone H2A type 1-B/E, ... (8 entities in total)
• Functional Keywords: telomeric dna, nucleosome core particle, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 10
• Total formula weight: 176474.08

Authors

Soman, A.,Liew, C.W.,Teo, H.L.,Berezhnoy, N.,Korolev, N.,Rhodes, D.,Nordenskiold, L. (deposition date: 2019-11-10, release date: 2020-04-22, Last modification date: 2023-11-22)

Primary citation

Soman, A.,Liew, C.W.,Teo, H.L.,Berezhnoy, N.V.,Olieric, V.,Korolev, N.,Rhodes, D.,Nordenskiold, L.
The human telomeric nucleosome displays distinct structural and dynamic properties.
Nucleic Acids Res., 48:5383-5396, 2020

PubMed Abstract: Telomeres protect the ends of our chromosomes and are key to maintaining genomic integrity during cell division and differentiation. However, our knowledge of telomeric chromatin and nucleosome structure at the molecular level is limited. Here, we aimed to define the structure, dynamics as well as properties in solution of the human telomeric nucleosome. We first determined the 2.2 Å crystal structure of a human telomeric nucleosome core particle (NCP) containing 145 bp DNA, which revealed the same helical path for the DNA as well as symmetric stretching in both halves of the NCP as that of the 145 bp '601' NCP. In solution, the telomeric nucleosome exhibited a less stable and a markedly more dynamic structure compared to NCPs containing DNA positioning sequences. These observations provide molecular insights into how telomeric DNA forms nucleosomes and chromatin and advance our understanding of the unique biological role of telomeres.

PubMed: 32374876
DOI: 10.1093/nar/gkaa289

Experimental method

X-RAY DIFFRACTION (2.6 Å)