6L9H
The Human Telomeric Nucleosome Displays Distinct Structural and Dynamic Properties
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-17 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.455, 109.388, 176.376 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.000 - 2.600 |
R-factor | 0.2384 |
Rwork | 0.236 |
R-free | 0.27790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lz0 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.760 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.961 | 2.899 |
High resolution limit [Å] | 2.518 | 2.518 |
Rmerge | 0.114 | 1.506 |
Rmeas | 0.119 | 1.567 |
Rpim | 0.033 | 0.426 |
Number of reflections | 33289 | 1665 |
<I/σ(I)> | 14.3 | |
Completeness [%] | 94.5 | 79.6 |
Redundancy | 13 | 13.4 |
CC(1/2) | 0.998 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293.15 | Manganase chloride, potassium chloride, potassium cacodylate, MPD and trehalose |