6KQB

A long chain secondary alcohol dehydrogenase of Micrococcus luteus

Summary for 6KQB

Entry DOI	10.2210/pdb6kqb/pdb
Related	6KQ9
Descriptor	3-hydroxybutyryl-CoA dehydrogenase (2 entities in total)
Functional Keywords	alcohol dehydrogenase, oxidoreductase
Biological source	Micrococcus luteus
Total number of polymer chains	2
Total formula weight	73715.62
Authors	Kim, H.J.,Kim, J.S. (deposition date: 2019-08-16, release date: 2020-08-19, Last modification date: 2023-11-22)
Primary citation	Seo, E.J.,Kim, H.J.,Kim, M.J.,Kim, J.S.,Park, J.B. Cofactor specificity engineering of a long-chain secondary alcohol dehydrogenase from Micrococcus luteus for redox-neutral biotransformation of fatty acids. Chem.Commun.(Camb.), 55:14462-14465, 2019 Cited by PubMed Abstract: Structure-based engineering of a NAD+-dependent secondary alcohol dehydrogenase from Micrococcus luteus led to a 1800-fold increase in catalytic efficiency for NADP+. Furthermore, the engineered enzymes (e.g., D37S/A38R/V39S/T15I) were successfully coupled to a NADPH-dependent Baeyer-Villiger monooxygenase from Pseudomonas putida KT2440 for redox-neutral biotransformations of C18 fatty acids into C9 chemicals. PubMed: 31728457 DOI: 10.1039/c9cc06447h PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.261 Å)

Structure validation