6KKO

The crystal structure of SiaB-SiaC complex from Pseudomonas aeruginosa

Summary for 6KKO

• Entry DOI: 10.2210/pdb6kko/pdb
• Descriptor: Putative serine phosphatase, DUF1987 domain-containing protein, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: siab-siac complex, pseudomonas aeruginosa, gene regulation
• Biological source: Pseudomonas aeruginosa; More
• Total number of polymer chains: 4
• Total formula weight: 70950.31

Authors

Gan, J.H.,Yang, C. (deposition date: 2019-07-26, release date: 2020-06-10, Last modification date: 2024-10-23)

Primary citation

Chen, G.K.,Gan, J.H.,Yang, C.,Zuo, Y.L.,Peng, J.,Li, M.,Huo, W.P.,Xie, Y.P.,Zhang, Y.N.,Wang, T.T.,Deng, X.,Liang, H.H.
The SiaA/B/C/D signaling network regulates biofilm formation in Pseudomonas aeruginosa.
Embo J., 39:e103412-e103412, 2020

PubMed Abstract: Bacterial cyclic-di-GMP (c-di-GMP) production is associated with biofilm development and the switch from acute to chronic infections. In Pseudomonas aeruginosa, the diguanylate cyclase (DGC) SiaD and phosphatase SiaA, which are co-transcribed as part of a siaABCD operon, are essential for cellular aggregation. However, the detailed functions of this operon and the relationships among its constituent genes are unknown. Here, we demonstrate that the siaABCD operon encodes for a signaling network that regulates SiaD enzymatic activity to control biofilm and aggregates formation. Through protein-protein interaction, SiaC promotes SiaD diguanylate cyclase activity. Biochemical and structural data revealed that SiaB is an unusual protein kinase that phosphorylates SiaC, whereas SiaA phosphatase can dephosphorylate SiaC. The phosphorylation state of SiaC is critical for its interaction with SiaD, which will switch on or off the DGC activity of SiaD and regulate c-di-GMP levels and subsequent virulence phenotypes. Collectively, our data provide insights into the molecular mechanisms underlying the modulation of DGC activity associated with chronic infections, which may facilitate the development of antimicrobial drugs.

PubMed: 32090355
DOI: 10.15252/embj.2019103412

Experimental method

X-RAY DIFFRACTION (2.099 Å)