6KKO
The crystal structure of SiaB-SiaC complex from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9725 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.618, 42.015, 96.584 |
Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
Resolution | 37.740 - 2.099 |
R-factor | 0.2338 |
Rwork | 0.232 |
R-free | 0.27770 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.740 | 2.230 |
High resolution limit [Å] | 2.099 | 2.099 |
Number of reflections | 35956 | 10896 |
<I/σ(I)> | 9.4 | 2 |
Completeness [%] | 98.7 | 97.5 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.997 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1M Bis-Tris pH 6.5, 25% PEG 3350 |