6KKO

The crystal structure of SiaB-SiaC complex from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9725
Spacegroup name	P 1 21 1
Unit cell lengths	75.618, 42.015, 96.584
Unit cell angles	90.00, 93.45, 90.00

Refinement procedure

Resolution	37.740 - 2.099
R-factor	0.2338
Rwork	0.232
R-free	0.27770
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.740	2.230
High resolution limit [Å]	2.099	2.099
Number of reflections	35956	10896
<I/σ(I)>	9.4	2
Completeness [%]	98.7	97.5
Redundancy	3.3	3.3
CC(1/2)	0.997	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M Bis-Tris pH 6.5, 25% PEG 3350