6KI9

Apo structure of FabMG, novel types of Enoyl-acyl carrier protein reductase

Summary for 6KI9

• Entry DOI: 10.2210/pdb6ki9/pdb
• Descriptor: FabMG, novel types of Enoyl-acyl carrier protein reductase, 1,2-ETHANEDIOL, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• Functional Keywords: enoyl-acyl carrier protein reductase, fabmg, oxidoreductase
• Biological source: uncultured bacterium
• Total number of polymer chains: 3
• Total formula weight: 149775.49

Authors

Kim, S.,Rhee, S. (deposition date: 2019-07-17, release date: 2020-05-20, Last modification date: 2024-03-27)

Primary citation

Kim, S.H.,Khan, R.,Choi, K.,Lee, S.W.,Rhee, S.
A triclosan-resistance protein from the soil metagenome is a novel enoyl-acyl carrier protein reductase: Structure-guided functional analysis.
Febs J., 287:4710-4728, 2020

PubMed Abstract: The synthetic biocide triclosan targets enoyl-acyl carrier protein reductase(s) (ENR) in bacterial type II fatty acid biosynthesis. Screening and sequence analyses of the triclosan resistome from the soil metagenome identified a variety of triclosan-resistance ENRs. Interestingly, the mode of triclosan resistance by one hypothetical protein was elusive, mainly due to a lack of sequence similarity with other proteins that mediate triclosan resistance. Here, we carried out a structure-based function prediction of the hypothetical protein, herein referred to as FabMG, and in vivo and in vitro functional analyses. The crystal structure of FabMG showed limited structural homology with FabG and FabI, which are also involved in type II fatty acid synthesis. In vivo complementation and in vitro activity assays indicated that FabMG is functionally a FabI-type ENR that employs NADH as a coenzyme. Variations in the sequence and structure of FabMG are likely responsible for inefficient binding of triclosan, resulting in triclosan resistance. These data unravel a previously uncharacterized FabMG, which is prevalent in various microbes in triclosan-contaminated environments and provide mechanistic insight into triclosan resistance.

PubMed: 32112503
DOI: 10.1111/febs.15267

Experimental method

X-RAY DIFFRACTION (1.64 Å)