6KBW

Crystal structure of Tmm from Myroides profundi D25

Summary for 6KBW

• Entry DOI: 10.2210/pdb6kbw/pdb
• Descriptor: Trimethylamine monooxygenase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total)
• Functional Keywords: flavin-containing monooxygenase, flavoprotein
• Biological source: Myroides profundi
• Total number of polymer chains: 2
• Total formula weight: 112923.31

Authors

Li, C.Y.,Zhang, Y.Z. (deposition date: 2019-06-26, release date: 2020-07-08, Last modification date: 2023-11-22)

Primary citation

Qin, Q.L.,Wang, Z.B.,Su, H.N.,Chen, X.L.,Miao, J.,Wang, X.J.,Li, C.Y.,Zhang, X.Y.,Li, P.Y.,Wang, M.,Fang, J.,Lidbury, I.,Zhang, W.,Zhang, X.H.,Yang, G.P.,Chen, Y.,Zhang, Y.Z.
Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage.
Sci Adv, 7:-, 2021

PubMed Abstract: High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. D25, obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine -oxide (TMAO) by a TMA monooxygenase, Tmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-Tmm pathway was further confirmed by introducing it into and Encoded TmaT-like and Tmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and Tmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.

PubMed: 33771875
DOI: 10.1126/sciadv.abf9941

Experimental method

X-RAY DIFFRACTION (1.686 Å)