6KBW
Crystal structure of Tmm from Myroides profundi D25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.543, 99.372, 144.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.956 - 1.686 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.20590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ipy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.118 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.960 | 1.780 |
| High resolution limit [Å] | 1.686 | 1.690 |
| Rmerge | 0.082 | 0.440 |
| Rmeas | 0.089 | |
| Rpim | 0.034 | 0.317 |
| Number of reflections | 115098 | 12333 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 93.4 | 69.9 |
| Redundancy | 11.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M Magnesium acetate 0.1M Mes, pH 6.5 10%(w/v) PEG 10000 |
