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6KBB

Role of the DEF/Y motif of Swc5 in histone H2A.Z deposition

Summary for 6KBB
Entry DOI10.2210/pdb6kbb/pdb
DescriptorHistone H2A type 1-D, Histone H2B type 2-E, SWR1-complex protein 5, ... (4 entities in total)
Functional Keywordshistone chaperone, transcription
Biological sourceHomo sapiens (Human)
More
Total number of polymer chains6
Total formula weight62107.62
Authors
Huang, Y.,Zhou, Z. (deposition date: 2019-06-24, release date: 2020-01-29, Last modification date: 2023-11-22)
Primary citationHuang, Y.,Sun, L.,Pierrakeas, L.,Dai, L.,Pan, L.,Luk, E.,Zhou, Z.
Role of a DEF/Y motif in histone H2A-H2B recognition and nucleosome editing.
Proc.Natl.Acad.Sci.USA, 117:3543-3550, 2020
Cited by
PubMed Abstract: The SWR complex edits the histone composition of nucleosomes at promoters to facilitate transcription by replacing the two nucleosomal H2A-H2B (A-B) dimers with H2A.Z-H2B (Z-B) dimers. Swc5, a subunit of SWR, binds to A-B dimers, but its role in the histone replacement reaction was unclear. In this study, we showed that Swc5 uses a tandem DEF/Y motif within an intrinsically disordered region to engage the A-B dimer. A 2.37-Å X-ray crystal structure of the histone binding domain of Swc5 in complex with an A-B dimer showed that consecutive acidic residues and flanking hydrophobic residues of Swc5 form a cap over the histones, excluding histone-DNA interaction. Mutations in Swc5 DEF/Y inhibited the nucleosome editing function of SWR in vitro. Swc5 DEF/Y interacts with histones in vivo, and the extent of this interaction is dependent on the remodeling ATPase of SWR, supporting a model in which Swc5 acts as a wedge to promote A-B dimer eviction. Given that DEF/Y motifs are found in other evolutionary unrelated chromatin regulators, this work provides the molecular basis for a general strategy used repeatedly during eukaryotic evolution to mobilize histones in various genomic functions.
PubMed: 32001508
DOI: 10.1073/pnas.1914313117
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.365 Å)
Structure validation

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